NANODRUG SEPARATION KIT
NANODRUG SEPARATION KIT
The detection of drug release is of importance for quality control and clinical development of nano drugs. Drug regulatory departments in various countries have definite requirements for the quantification of loaded drugs and free drugs in studies of encapsulation efficiency, in vitro dissolution or release, and pharmacokinetics of nanodrugs.

Functional

Mild Separation Condition

Low speed centrifugation (2000 × g) drugs can completely separate loaded from free drugs, without damaging nanoparticles (such as liposomes) and causing drug leakage.

High Responsbilty to Peglated Nano Drugs

It can still be effectively separated even if the PEG content of nano drugs is as low as 1% or diluted multiple times.

Excellent Separation Effect

The results are more accurate with lower deviation comparing to solid-phase extraction (SPE). The advantages are more obvious when the free drug content is low.

Strong Anti-interference Ability

The separation effect is not interfered by blood protein and anticoagulant in the blood sample.

High experimental efficiency

Simultaneous processing of a large number of samples is achieved with simple centrifugation process.
Fig. Doxil preserves integrity after SR induced aggregation, and can be recovered with excessive free PEG
Fig. The sedimentation efficiency for liposomes with different PEG contents and concentration is unchanged
Fig. Comparison of separation results of doxorubicin liposomes with SR or SPE methods
Fig. The results of SR separation of Doxil are not affected by mouse or human serum or added Anticoagulant

Working Principle

The separation kit can disrupt hydration layer of pegylated nanodrugs by combination between the separation reagent (SR) and the PEG on the surface of nanodrugs, promoting theaggregation and sedimentation of pegylated nanodrugs, realizing the separation of loaded and free drugs through low-speed centrifugation.

 

 

Fig. Working principle of nano drug separation reagent (left) and sample state during liposome separation process (right)

SOP

Step1
Take the required separation reagents (1 microtube per sample) and melt them in an ice water bath. If there is liquid hanging on the tube wall, centrifuge it briefly at low speed to allow the liquid to flow down the tube wall.
Step2
The sample (volume between 25 and 125 μL is recommended) add to the microtube, pipitte up and down several times (not to produce bubbles during operation). Incubate in an ice water bath for 30 minutes.
Step3
Centrifuge the microtube (2000 g, 4 ℃, 15 min), the precipitate is the nano drug (loaded drug), while he free drug is in the supernatant.

TIPS

The reagent kit is suitable for the separation of peglated nano drugs (excluding PEG polymer micelles).

The reagent kit is stored at -20 ℃ and transported through cold-chain. Immediately use after melting and do not freeze repeatedly.